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BioToolKit is a collection of 45 software tools frequently used by bench biomedical scientists. It is designed by bench scientists and easy to use. Functions include PCR primer design (standard, multiplex, degenerate, MSP, DGGE, gene synthesis, ...), antibody design, siRNA design, statistical tools (datafit for ic50, SVM learning, ...), a large number of calculators (centrifugation force, OD, molecular weight, units conversions, ...), label printing and grade book.